A Study on the Formation of Liquid Ordered Phase in Lysophospholipid/Cholesterol/1,3-Butanediol/ Water and Lysophospholipid/Ceramide/1,3- Butanediol/Water Systems

Abstract

Formation of a liquid ordered phase in lysophospholipid/cholesterol/1,3-butanediol/water and lysophospholipid/ceramide/1,3-butanediol/water systems was investigated. Differential scanning calorimetry confirmed that a liquid ordered phase was formed in the lysophospholipid/cholesterol/1,3-butanediol/water system similar to that in phospholipid systems. The structure of liquid ordered phase was analyzed by using X-ray scattering measurements. It was revealed that the liquid ordered phase has a lamellar structure in which the hydrophobic chains are less ordered than in α-type crystals. On the other hand, in the lysophospholipid/ceramide/1,3-butanediol/water system, a liquid ordered phase was not formed.