Journal of Oleo Science
Online ISSN : 1347-3352
Print ISSN : 1345-8957
ISSN-L : 1345-8957
Oils and Fats
Fatty Acid and Lipid Class Composition of the Microalga Phaeodactylum tricornutum
Yu-Hong YangLei DuMasashi HosokawaKazuo MiyashitaYume KokubunHisayoshi AraiHiroyuki Taroda
Author information
JOURNAL FREE ACCESS

2017 Volume 66 Issue 4 Pages 363-368

Details
Abstract

n-3 Polyunsaturated fatty acids (PUFAs) are essential for the prevention and/or risk reduction of some diseases, including cardiovascular diseases and cancer. Therefore, PUFAs-rich marine microalgae have received considerable research attention. The diatom Phaeodactylum tricornutum, a rich source of lipids and fucoxanthin, has commercial applications in the food and pharmaceutical industries. In this study, the lipid class composition and fatty acid distribution in P. tricornutum under high-density and scale-up cultivation were investigated by thin-layer chromatography and gas chromatography. The fucoxanthin content was quantified by reverse-phase high-performance liquid chromatography. Both total lipid and fucoxanthin contents were high, accounting for 321.89 and 4.47 mg/g dry weight, respectively. Neutral lipids were the major lipids, and triacylglycerol was the predominant neutral lipids (33.63% of total lipids). The glycolipids (GLs) and phospholipids (PLs) represented 20.95% and 31.39% of total lipids, respectively. Sulfoquinovosyl diacylglycerol (SQDG) was the largest GLs fraction (9.81% of total lipids). Phosphatidylglycerol (PG) was the major PLs and accounted for 13.53% of total lipids. The main fatty acids were hexadecanoic acid (C16:0), palmitoleic acid (C16:1), and eicosapentaenoic acid. In addition, docosahexaenoic acid only accumulated in the PLs fraction. These findings provide new information on the lipid types, fatty acid composition of each lipid class, and the fucoxanthin content in P. tricornutum.

Content from these authors
© 2017 by Japan Oil Chemists' Society
Previous article Next article
feedback
Top