Effect of Various Factors on Orange II Decoloration Reaction by Horseradish Peroxidase in the Presence of Detergent Enzymes

Abstract

The effects of four detergent enzymes on the Orange II decoloration reaction by horseradish peroxidase (HRP) were investigated. Stainzyme, Lipex, Celluclean, and Savinase did not affect the decoloration reaction up to a concentration of 0.1% Under weak alkaline to alkaline conditions, the presence of four enzymes did not affect the decoloration reaction, but affected the decoloration reaction under neutral to acidic conditions. The effect of Ca²⁺ and Mg²⁺ concentrations on Orange II decoloration reactions in the presence of four enzymes was also investigated. Ca²⁺ and Mg²⁺ did not affect the decoloration reaction up to 500 ppm.