Synchrotron FTIR Micro-spectroscopy Studies Demonstrate the Release of Ceramide Molecules from a Ceramide-containing UV-curable Acrylic Adhesive Resin

Abstract

A recent study has shown that the addition of ceramide to adhesive materials of medical sheets or tapes improves or maintains skin barrier functions despite the irritation and damage caused by the repeated removal of the sheet or tape. This fact may imply that ceramide molecules are released from the adhesive material and penetrate the skin. In this study, we investigated whether ceramide molecules are released from a UV-curable acrylic adhesive resin (acResin^®) sheet containing ceramide molecules attached to a cultured skin sample by estimating the local ceramide concentration from the data obtained by synchrotron Fourier transform infrared micro-spectroscopy analysis. Since section samples of uniform thickness could not be prepared, the intensity data of the amide I peak originating from ceramide were normalized by using the intensity data of the ester peak originating from the resin matrix. This analysis enabled the quantification of the change in local ceramide concentrations in the sheet samples. This result indicates that ceramide molecules were released from the acResin^® sheet attached to the cultured skin sheet.