Antioxidant and Anti-fatigue Activities of Seeds Oil of Ribes stenocarpum Maxim. from the Qinghai-Tibet Plateau

Abstract

Ribes stenocarpum Maxim. serves as raw material to optimize the supercritical CO₂ extraction process of seeds oil by response surface methodology. The components of seeds oil of Ribes stenocarpum Maxim. (RSO) were analyzed by gas chromatography (GC), and its antioxidation and anti-fatigue activities were also investigated. The findings indicated that the maximum oil yield of Ribes stenocarpum Maxim. by SC-CO₂ was 23.59% under the conditions of 38.0°C, 180 min, 32.0 MPa, 5.0 mL/min CO₂ flow rate. GC analysis showed that unsaturated fatty acid content of RSO was 85.08%. In vitro antioxidant assays demonstrated that RSO had the most potent hydroxyl radical scavenging activity, achieving 90% at a dosage of 0.9%. The anti-fatigue activity assessment showed that RSO could significantly prolong the forced swimming time of mice, decrease the levels of lactic acid (LD), blood urea nitrogen (BUN) and liver malondialdehyde (MDA), and increase the activities of lactate dehydrogenase (LDH), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) in liver tissue. The results of the study demonstrated that RSO has the ability to enhance glycogen storage in the body, enhance antioxidant capacity, and provide notable anti-fatigue activity.

graphical abstract