Two-stage Enzymatic Hydrolysis of Soybean Concentrate Phospholipid to Prepare Glycerylphosphorylcholine: Optimized by Response Surface Methodology

Abstract

A two-stage enzymatic hydrolysis method, in which phospholipase A₁ (PLA₁) was added after phospholipase A₂ (PLA₂) was added for a certain time, was successfully carried out to prepare glycerylphosphorylcholine (GPC) from soybean concentrated phospholipid. Effects of reaction variables on hydrolysis reaction were optimized using response surface methodology, and the optimal conditions were as follows: PLA₂ load of 1.25%, PLA₁ load of 0.70%, substrate concentration of 13%, reaction temperature of 41°C, and stirring rate of 680 rpm. Under the optimal conditions, the GPC yield reached 83.07%, which is close to the predicted value by the fitted model. This paper not only provides an efficient and low-cost method to prepare GPC, but also improves the high-value utilization of soybean concentrated phospholipid.