Abstract
The fundamental studies concerning the practical use of the microbial lipases have been reported in our laboratory. The availability of the lipases for determining the fatty acid dustribution in natural triglycerides (TG) was investigated.
Rhizopus delemar lipase was found to be the most specific enzyme for both the nature of fatty acid and the positions of fatty acid in triglyceride. The condition of the reaction for TG hydrolysis was established.
In this paper, we described the preferential hydrolysis of the primary ester linkage by the enzyme, the separation of the hydrolysis products by TLC, and the determination of the released fatty acids and the remained fatty acid in monoglyceride by GLC. From these results, it was confirmed that the Rhizopus delemer lipase could be available for analyzing most of natural vegetable oils and fats.
