Three 2-amino-1, 3-alkanediols (alkane : decane, dodecane, and tetradecane) (4) were synthesized by the catalytic reduction of sodium salts of
aci-2-nitro-1, 3-alkanediols, (3), in the presence of 5% Pd-C in glacial acetic acid at 40°C in good yields. Silylation of (4) with a mixture of HMDS and TMCS gave three compounds,
O, O-bis-TMS derivative of (4) [(5)], and
N, O, O-tris-TMS deriv-atives of
threo- and
erythro- (4) [
threo- and
erythro- (6)], but two ones,
threo- and
erythro- (6), in the case of BSA. The structures of (6) were confirmed by mass spectra. The characteristic fragment ions were
m/e=204 [base peak ion : formed by the cleveage between C-2 and C-3], M-105, M-103 and M-15. The
m/e values of these fragment ions were 72 larger than those of (5). Evidence for a facile determination of
erythro/threo ratios of (4) by GLC was demonstrated.
Erythro contents of (4) were ca. 70% in each case, in agreement with those calculated from the GLC of
O, O-bis-TMS derivatives of
N-acetyl- (4). Attempted separation of
erythro and
threo isomers of (4) were carried out by recrystallization of
N, O, O-triacetyl-2-amino-1, 3-alkanediols (7) and
N-acetyl-2-amino-1, 3-alkanediols (8).
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