Abstract
Reverse phase HPLC of various fatty acid esters has been studied using 050% H2O-MeOH as eluent. The esters were detected at 200210nm. The plots of log (corrected retention volume : V) vs. carbon numbers (CNs) of acid and alcohol residues in saturated esters gave good linear relationships. The slopes (Δlog V/ΔCN) of log V vs. CN of esters plots increased linearly with increasing the water content in eluent. Plots of area ratio vs. weight ratio of unsaturated methyl ester/12 : 0-Me gave good linear relationships, demonstrating a possibility of quantitation of fatty acid esters by reverse phase HPLC.
