On the Determination of Lipolytic Activity of Lipase by Surfactant Non-addition Method

Bioreactors for Fats and Fatty Acid Derivatives, Part XII

Abstract

In the determination of the lipolytic activity of lipase by surfactant non-addition method, attempts were made to broaden the range of proportionality between the volume of titration and amount of enzyme added to the assay mixture and to decrease the variation in replicate measurement data (precision). The introduction of baffle plates into the reaction vessel provided some improvement in both these respects. Increased agitation from 500rpm to 1000rpm was also effective for greater proportionality.