Abstract
The growth of Candida rugosa JF-101 was either stimulated or not affected by Tween 80 in n-decane (n-C10)n-dodecane (n-C12) media and inhibited in n-C13n-C16 media. The growth of Saccharomycopsis lipolytica IAM 4947 was stimulated by the surfactant in n-C10n-C13 and inhibited in n-C14n-C16. Dodecyl poly (oxyethylene) ether (C12POE10) strongly inhibited the growth of C. rugosa JF-101 in glucose medium and slightly so in n-alkane medium. C16POE20, C16POE30 and C18POE20 strongly inhibited its growth in n-C16 medium but failed to do so in glucose or n-C10 media. Several alcohols corresponding to CnPOE0, were also assessed for their ability to promote or inhibit growth. 1-Decanol and 1-dodecanol inhibited growth in glucose but not so in n-alkane. C. rugosa JF-101 and S. lipolytica IAM 4947, all having strong n-alkane-assimilating activity, showed greater affinity for n-C16 than C. tropicalis IAM 4826 having weak activity. The affinity of C. rugosa JF-101 was reduced 76% by Pronase but its respiratory activity (RA) did not decreased significantly. Speroplast of the yeast lost almost all RA on n-alkane and it subsequently could hardly be recovered by Tween 80.
