Abstract
High-performance liquid chromatographic separation of diacid-1, 2-diacylglycerol isomers having different acyl groups in reversed positions as their 3, 5-dinitrobenzoates were carried out on a silver-loaded ion exchange column prepared by Christie's method using mixtures of n-hexane/1, 2-dichloroethane/ethanol as the mobile phase. Separation of diacylglycerol (DG) reversed isomers synthesized from palmitic, oleic, linoleic and linolenic acids with glycerol (1-16 : 0, 2-18 : 1-and 1-18 : 1, 2-16 : 0-DGs), (1-16 : 0, 2-18 : 2-and 1-18 : 2, 2-16 : 0-DGs), and (1-16 : 0, 2-18 : 3- and 1-18 : 3, 2-16 : 0-DGs) were achieved.
