Abstract
A high-performance liquid chromatographic method with high sensitivity and selectivity for analysis of anionic surfactants (ASF) is described. ASF such as alkylbenzenesulfonate (LAS), alkanesulfonate (SAS), α-olefinsulfonate (AOS), alkyl sulfate (AS) and poly (oxyethylene) alkyl ether sulfate (ES) were separated by reversed-phase chromatography using ODS-silica (Hitachi gel 3053) as a stationary phase and 1mM sodium perchlorate in acetonitrile-water (30 : 70) as a mobile phase and were detected with non-suppressor type conductive detector. The calibration curve was linear in the range from 0.005 to 17μg for C12 AS-Na and the relative standard deviation with five measurements was 0.31% for 1.0μg C12 AS-Na. The results of homolog analysis of LAS by the proposed method showed good agreement with those by the UV detection. Nonionic, cationic and amphoteric surfactants did not interfere with the analysis of ASF. By the proposed method, ASF in commercial household and cosmetic products were analyzed with high sensitivity and selectivity. Better separations were achieved by the gradient elution method.
