Abstract
Optimum conditions were determined for the preparation of phosphatidylethanolamine (PE), phosphatidyl-N-methylethanolamine (PMME), and phosphatidyl-N, N-dimethylethanolamine (PDME) from phosphatidylcholine (PC) concentrated soy phospholipid (PC-90) by trans-phosphatidylation catalyzed by phospholipase D : PC-90 contaminated with PE (10 %); 100 mg, aminoalcohol ; 3 M, phospholipase D ; 1.5 IU, calcium ion ; 1 M, diethyl ether ; 5 mL, water content ; 12 mL, pH ; 4.85.2, and reaction time ; 30 min. Under these conditions, conversion from PC-90 to PE, PMME, and PDME was 95, 83, and 74 %, respectively. Differences in these values are considered due to the specificity of phospholipase D for each aminoalcohol.
