1995 Volume 44 Issue 3 Pages 197-202
The phospholipid content of Euglena cells and changes in lipid components during the preservation of harvested cells were investigated. Analysis of phospholipid content of cells cultured under heterotrophic conditions in the dark indicated phosphatidylinositol as well as phosphatidylcholine to be the major components of pospholipid, phosphatidylserine to be absent.
When cells were aseptically preserved at 25°C for 7 d, the phospholipid fraction decreased and free fatty acid fraction increased, possibly due to phospholipase reactions. Phospholipase activity was thus determined using phosphatidylcholine, which markedly decreased during the course of preservation, as the substrate. It was thus possible to detect phospholipase A, C, and D activity.
