Abstract
A method for the convenient quantitative determination of hydrogenated phosphatidylcholine (PC), for use as a cosmetic ingredient, was developed by using high performance liquid chromatography (HPLC), equipped with a silica gel column (Senshu Scientific, AQUASIL SS-352 N) and a UV detector (205 nm). This column possesses not only the usual properties of a silica gel column but can accommodate a mobile phase containing water. In this study, the solution used as the mobile phase was methanol/0.01 M potassium dihydrogen-phosphate, citric acid buffer (pH 3.0) (95/5, by vol.) at 1.0 mL/min. Hydrogenated PC could be detected rapidly, and there was no interfering peak. Non-hydrogenated PC was also present. Linear response was noted for hydrogenated and non-hydrogenated PC at 1004000 μg/mL and 52000 μg/mL, respectively. Minimum quantification was 50 and 1 μg/mL for these samples. Theretention time of hydrogenated PC was dependent on carbon chain length of fatty acid in PC molecules.
Other phospholipids such as phosphatidylinositol (PI), phosphatidylethanolamine (PE), lysophosphatidylcholine (LPC), and sphingomyelin (SPH) could also be determined independently by this method.
