2014 年 130 巻 6 号 p. 225-230
In order to develop the“ Bio-filter”, silicon nitride porous bodies with micro-macro complex pore structure were fabricated using the tape casting process. Using Saccharomyces cerevisiae and Lactobacillus casei as the aerobic and anaerobic model microbes, aqueous solutions containing these microbes were passed through the porous bodies for 72 hrs. Then, the aqueous solution of 1% glucose saturated with oxygen in air was passed through them. It was confirmed that both microbes were simultaneously fixed on the surface of the macro-pores with the formation of the bio-film, suggesting the successful formation of the microbe consortium. In the case that only Saccharomyces cerevisiae was fixed on the surface of the macro-pores, the formation of the bio-film was hardly observed. In contrast, when only Lactobacillus casei was used, the network structure of the bio-film was observed inside the micro-pores between the macro pores. The pH values and the kinds and concentrations of the products contained in the effluents were different for these three cases. It was found that the concentration of lactic acid was much larger in the case that two microbes were simultaneously fixed than in the case that only Lactobacillus casei was fixed, suggesting the successful formation of the anaerobic condition through the oxygen consumption by the aerobic metabolism of Saccharomyces cerevisiae.