Protocol for phosphoproteomic analysis

Abstract

Protein phosphorylation is one of the most major post-translational modifications which is involved in regulation of various cellular functions. The number of phosphorylation sites identified has dramatically increased with the development of phosphoproteome analysis technology by mass spectrometry. The remarkable development of this technology has been supported mainly by the development and improvement of pretreatment methods such as phosphopeptide enrichment, the evolution of mass spectrometers, and the development of data analysis methods and software. In this paper we focus on pretreatment methods and present an example of clinical phosphoproteome analysis with limited sample amounts.