Responses to Neonicotinoids of Chicken α7 Nicotinic Acetylcholine Receptors: Effects of Mutations of Isoleucine 191 in Loop F to Aromatic Residues

抄録

The effects on the responses to neonicotinoids and related nicotinic agonists of three site-directed mutations (I191W, I191F and I191Y) in loop F of the acetylcholine-binding site were studied using the chicken α7 nicotinic acetylcholine receptor (nAChR) expressed in Xenopus laevis oocytes. Voltage-clamp electrophysiology was employed to show that, whereas the I191F mutation scarcely affected the concentration-response curves for neonicotinoids, the I191W mutation increased the maximum amplitude of responses to these ligands. By contrast, the I191Y mutation reduced the maximum amplitude of responses of the α7 nAChR to the insecticides. © Pesticide Science Society of Japan