Synthesis of photolabile Δlac-acetogenin for photoaffinity labeling of mitochondrial complex I

Masatoshi Murai; Naoya Ichimaru; Masato Abe; Takaaki Nishioka; Hideto Miyoshi

doi:10.1584/jpestics.31.156

Notes

Synthesis of photolabile Δlac-acetogenin for photoaffinity labeling of mitochondrial complex I

Masatoshi Murai, Naoya Ichimaru, Masato Abe, Takaaki Nishioka, Hideto Miyoshi

著者情報

キーワード: complex I, acetogenin, respiratory inhibitor

ジャーナルフリー

2006 年 31 巻 2 号 p. 156-158

DOI https://doi.org/10.1584/jpestics.31.156

詳細

抄録

Δlac-Acetogenins are a novel type of inhibitor acting at the terminal electron transfer step of mitochondrial NADH-ubiquinone oxidoreductase (complex I). To identify the binding site of Δlac-acetogenins by photoaffinity labeling, we synthesized a photolabile Δlac-acetogenin that possesses a biotin probe to enable the detection and the isolation of the labeled peptide without the use of a radioisotope. The photolabile Δlac-acetogenin synthesized in this study elicited potent inhibition of bovine heart mitochondrial complex I at the nanomolar level. © Pesticide Science Society of Japan

責任著者(Corresponding author)

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