Abstract
To detect small amount of oxamyl, indirect competitive enzyme-linked immunosorbent assays. (indirect C-ELISAs) were established. Four haptens for immunogen and three haptens for coating antigen on indirect C-ELISA were synthesized and their polyclonal (PoAbs) and monoclonal antibodies (MoAbs) were prepared. Hapten N, N-dimethyl-2-(5-carboxypentyl) carbamoyloxyimino-2-(methylthio) acetamide was the most effective to raise antibodies toward oxamyl out of haptens for immunogen. MoAbs prepared with this hapten were more highly reactive than PoAbs. In particular, MoAb OXM 6-8 showed the highest reactivity. When N, N-dimethyl-2-(4-carboxycyclohexyl) carbamoyloxyimino-2-(methylthio) acetamide was used for coating antigen on indirect C-ELISA with OXM 6-8, the highest sensitivity was observed and the concentration for 50% inhibition value showed 3.6ng/ml. Methanol extract of potato showed no effect on the reactivity.
