Abstract
From electron microscopic observation of the mycelia of Pyricularia oryzae treated with didodecyldimethylammonium bromide, electron-dense substance outside cell membrane, enlarged nuclear membrane, disappearance of cell wall structure, and collapsed cell were found. As to leakage of the cell component, optical density at 260nm of the incubation solution of P. oryzae was highest in the case of 100ppm rather than 50ppm and 300ppm treatment. This compound remarkably inhibited protein synthesis of P. oryzae, and inhibition of uptake of the substance and leakage of incorporated material were observed. It seems that cationic surfactants act on the cell membrane, and cause degeneration of cell membrane, inhibition of transport function, and subsequent inhibition of protein and lipid synthesis.
