Effect of Overexpression of the Brain-Specific Na⁺/Ca²⁺ Exchanger Splice Variant NCX1.5 on NO Cytotoxicity in HEK293 Cells

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Nitric oxide (NO) induces cytotoxicity in neuronal and glial cells via activation of the Na⁺/Ca²⁺ exchanger (NCX). This study examined the role of the predominant brain-specific NCX splice variant NCX1.5 in NO-induced cytotoxicity in the HEK293 cell expression system. Cells were transfected with the plasmid construct pcDNA3.1/V5-His containing full-length rat NCX1.5 cDNA. There was no difference in the cytotoxic effects of the NO donors sodium nitroprusside and S-nitroso-N-acetylpenicillamine between control and transfected cells. These results suggest that NO cytotoxicity is not dependent on NCX1.5.