Effect of Overexpression of the Brain-Specific Na+/Ca2+ Exchanger Splice Variant NCX1.5 on NO Cytotoxicity in HEK293 Cells

Yuki Ota; Takuya Kawanai; Ryo Watanabe; Akira Nishimura; Yukio Ago; Kazuhiro Takuma; Toshio Matsuda

doi:10.1254/jphs.13012SC

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Effect of Overexpression of the Brain-Specific Na⁺/Ca²⁺ Exchanger Splice Variant NCX1.5 on NO Cytotoxicity in HEK293 Cells

Yuki Ota, Takuya Kawanai, Ryo Watanabe, Akira Nishimura, Yukio Ago, Kazuhiro Takuma, Toshio Matsuda

著者情報

キーワード: Na⁺/Ca²⁺ exchanger, nitric oxide, cytotoxicity

ジャーナルフリー早期公開

論文ID: 13012SC

DOI https://doi.org/10.1254/jphs.13012SC

この記事には本公開記事があります。

The final version of this article is now available: Vol. 121 (2013), No. 4 pp. 351-354

詳細

抄録

Nitric oxide (NO) induces cytotoxicity in neuronal and glial cells via activation of the Na⁺/Ca²⁺ exchanger (NCX). This study examined the role of the predominant brain-specific NCX splice variant NCX1.5 in NO-induced cytotoxicity in the HEK293 cell expression system. Cells were transfected with the plasmid construct pcDNA3.1/V5-His containing full-length rat NCX1.5 cDNA. There was no difference in the cytotoxic effects of the NO donors sodium nitroprusside and S-nitroso-N-acetylpenicillamine between control and transfected cells. These results suggest that NO cytotoxicity is not dependent on NCX1.5.

責任著者(Corresponding author)

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