Tumor Necrosis Factor-α Mediates the Blood–Brain Barrier  Dysfunction Induced by Activated Microglia in Mouse Brain  Microvascular Endothelial Cells

Tsuyoshi Nishioku; Junichi Matsumoto; Shinya Dohgu; Noriko Sumi; Kenji Miyao; Fuyuko Takata; Hideki Shuto; Atsushi Yamauchi; Yasufumi Kataoka

doi:10.1254/jphs.09292SC

この記事には本公開記事があります。本公開記事を参照してください。
引用する場合も本公開記事を引用してください。

Tumor Necrosis Factor-α Mediates the Blood–Brain Barrier Dysfunction Induced by Activated Microglia in Mouse Brain Microvascular Endothelial Cells

Tsuyoshi Nishioku, Junichi Matsumoto, Shinya Dohgu, Noriko Sumi, Kenji Miyao, Fuyuko Takata, Hideki Shuto, Atsushi Yamauchi, Yasufumi Kataoka

著者情報

キーワード: microglia, tumor necrosis factor-α (TNF-α), blood–brain barrier

ジャーナルフリー早期公開

論文ID: 09292SC

DOI https://doi.org/10.1254/jphs.09292SC

この記事には本公開記事があります。

The final version of this article is now available: Vol. 112 (2010) , No. 2 p.251

詳細

抄録

The present study was designed to elucidate the involvement of tumor necrosis factor-α (TNF-α) release from activated microglia in the induction of blood–brain barrier (BBB) dysfunction in an in vitro co-culture system with mouse brain microvascular endothelial cells (MBEC4) and microglia. Lipopolysaccharide (LPS)–activated microglia increased the permeability of MBEC4 cells to sodium-fluorescein, and this hyper-permeability was blocked by a neutralizing antibody against TNF-α. LPS stimulated microglia to facilitate TNF-α release. These findings suggested that TNF-α released from activated microglia is attributable to BBB dysfunction.

責任著者(Corresponding author)

J-STAGEへの登録はこちら（無料）