Cellular Ca2+ Dynamics in Urinary Bladder Smooth Muscle From Transgenic Mice Overexpressing Na+-Ca2+ Exchanger

Hidemichi Murata; Shingo Hotta; Eiji Sawada; Hisao Yamamura; Susumu Ohya; Satomi Kita; Takahiro Iwamoto; Yuji Imaizumi

doi:10.1254/jphs.09319SC

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Cellular Ca²⁺ Dynamics in Urinary Bladder Smooth Muscle From Transgenic Mice Overexpressing Na⁺-Ca²⁺ Exchanger

Hidemichi Murata, Shingo Hotta, Eiji Sawada, Hisao Yamamura, Susumu Ohya, Satomi Kita, Takahiro Iwamoto, Yuji Imaizumi

著者情報

キーワード: Na⁺-Ca²⁺ exchanger, urinary bladder smooth muscle, transgenic mouse

ジャーナルフリー早期公開

論文ID: 09319SC

DOI https://doi.org/10.1254/jphs.09319SC

この記事には本公開記事があります。

The final version of this article is now available: Vol. 112 (2010) , No. 3 p.373

詳細

抄録

The rise of Ca²⁺ concentration ([Ca²⁺]_i) by reducing external Na⁺ in urinary bladder smooth muscle cells (UBSMCs) from transgenic mice overexpressing Na⁺/Ca²⁺ exchanger type-1.3 (NCX1.3^tg/tg) was about 4 times as large as that in the wild-type (WT). NCX1 protein expression in UB increased about 4-fold in NCX1.3^tg/tg. The Ca²⁺ release by caffeine in UBSMCs was comparable between NCX1.3^tg/tg and WT, but [Ca²⁺]_i decay was faster in NCX1.3^tg/tg. Contractions induced by acetylcholine, 60 mM K⁺, or electrical stimulation were significantly smaller in UB segments of NCX1.3^tg/tg. NCX worked in Ca²⁺-extrusion mode during these contractions in UBSMCs of both WT and NCX1.3^tg/tg.

責任著者(Corresponding author)

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