The Japanese Journal of Pharmacology
Online ISSN : 1347-3506
Print ISSN : 0021-5198
ISSN-L : 0021-5198
A New Simple Plastic Chemotaxis Device of the Boyden Chamber Type Utilizing an Immunoassay Plate
Kazuyoshi WATANABEHideo NAKAGAWASusumu TSURUFUJI
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ジャーナル フリー

1985 年 39 巻 1 号 p. 102-104

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抄録
A new simple and economical plastic Boyden chamber for in vitro assay of leukocytes chemotaxis was devised by the use of a commercially available immunoassay plate. Zymosan-activated rat serum, formly-methionyl-leucylphenylalanine and leukotriene B4 caused concentration-dependent migration of the leukocytes into the lower chamber. About 50% of the cells loaded in the upper chamber migrated into the lower chamber for 80 min at the optimal concentrations of the three chemoattractants tested.
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© The Japanese PharmacologicalSociety
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