クロマツの卵細胞内に存在する蛋白胞の細胞化学的研究

抄録

1. The “proteid vacuoles” in the cytoplasm of egg cell of Pinus thunbergii were studied with staining methods.
2. The proteid vacuoles first appear in the central cell. With maturation of the egg cell, they increase more and more in number and in their stainability by Heidenhain's iron-hematoxylin and protein-staining reactions. The number and the stainability of them reach the maximum state just before fertilization. After fertilization they begin to decrease in number and nearly completely disappear by the time of proembryo formation at the base of the egg cell.
3. The proteid vacuoles contain not only protein but also PNA and a little polysaccharides.
4. In all the stages of oogenesis, the proteid vacuoles are separated into three groups by centrifuge treatment; the first of them are moved toward the centrifugal end, the second toward the centripetal end and the remaining ones are distributed in the middle part of the cell. Those gathered at the centripetal end under centrifugal force are propable to contain lipids.
5. The nutritive significance of the proteid vacuoles in the maturation of egg cell and in the early development of proembryo is discussed.