Effect of Lupinus Growth Factor on the in vitro Growth of Embryos of Various Plants and Carrot Root Tissue

Abstract

1.The optimum concentration of Lupinus diffusate, which contanis growth-promoting factor for young Datura embryo in vitro, is equivalent to 10g of Lupinus seed/100ml of the medium.
The growth-promoting factor in the Lupinus diffusate is not destroyed by autoclaving at 1.0kg/cm² overpressure for ten minutes.
The Lupinus diffusate promotes remarkably the growth of young Datura embryos, but not the old ones.
2. By Lupinus diffusate, the growth of embryos of Pharbitis nil, Bidens biternata, Brassica campestris, Lupinus luteus, Capsella bursa-pastoris and of hybrid embryos from a genetically incompatible cross of Brasssca pekinensis×B. chinensis are promoted remarkably. This diffusate is slightly effective for the growth of embryos of Stellaria media, Antirrhinum majus, Astragalus sinicus, and Triticum aestivum, nearly ineffective to that of Vicia faba and V. sativa, and inhibitory to Iris pseudoacorus.
3. In Stellaria and Triticum, the Lupinus diffusate supports embryonal growth and prevents precocious germination.
4. The Lupinus diffusate promoted the growth of cultured callus tissue of carrot root.
5. Similar embryo factor activity was found in the ethanol diffusates of seeds of Glycine soja, Phaseolus vulgaris var. humilis and “Black Valentine”. Arachis hypogaea, Pisum sativum, Canavalia ensiformis and of fruits of Brassica campestris.