エリスロポエチンの血小板細胞内遊離カルシウム濃度に及ぼす影響－健常者,高血圧者,腎不全患者での検討－
1994 年 36 巻 3 号 p. 250-258
詳細
1994 年 36 巻 3 号 p. 250-258
Intracellular free calcium concentration ( [Ca2+] i) was examined in the platelets of 15 control subjects (NT), 6 predialysis patients with chronic renal failure (CRF), 17 patients on hemodialysis (HD), 20 patients on continuous ambulatory peritoneal dialysis (CAPD), 10 normotensive persons with genetic hypertension (GHT) and 8 essential hypertensive patients (EHT). Levels of (Ca2+) i in the platelets were measured by the fluorescent calcium indicator Fura-2. Resting (Ca2+) i in CRF and HD patients was higher than the value in NT and that in CAPD patients was similar to NT. rHuEPO significantly increased the level of (Ca2+) i in CRF and HD patients compared to those in NT. Under resting and EPO-stimulated conditions, the levels of (Ca2+) i in GHT and EHT were higher than those in NT. rHuEPO increased the levels of (Ca2+) i in the absence of extracellular calcium in NT, GHT and EHT. In addition, EPO-stimulated calcium influx in GHT and EHT was greater than that in NT. Thus, it appears that the mechanism of rHuEPO-induced hypertension may be mainly due to elevation of (Ca2+) i. EPO may contribute to the development of genetic hypertension.
