抄録
Leishmania spp. , the agents of human visceral, post kala-azar dermal and cutaneous leishmaniasis, are the intracellular parasites that must be recognised and internalised by host macrophages for its pathogenesis. The involvement of specific ligand-receptor interaction necessary for the entry of different Leishmania promastigotes into murine macrophages were investigated. The effect of two enzymes on the attachment and uptake of Leishmania promastigotes by macrophages which might be found in the wound caused by the bite of the phlebotomine sandfly vector were also examined. With the two sugars tested, mannose and galactose were produced a significant inhibition of uptake in viscerotropic and dermotropic form of leishmaniasis, respectively. Mild trypsinization inhibited the uptake potential in all cases, with much higher value in the case of post kala-azar dermal leishmaniasis. Treatment of neuraminidase to macrophages enhanced attachment in all three strains to some extent. These results indicate that the nearly similar type of inhibition profile of dermal and cutaneous strains were probably due to the similar type of skin habitat.
