1994 年 4 巻 2 号 p. 71-82
Six species of insect trypanosomatids of the genus Crithidia were analyzed using different biochemical and molecular biology techniques, such as kinetoplast DNA(kDNA) restriction pattern analysis, kDNA cross-hybridizations, zymodeme analysis and, computer analysis of kDNA minicircle sequences for C. fasciculata and C. oncopelti. Our results indicate that C. deanei andC. oncopelti have widely diverged from the other species we analyzed, since their kDNA did not cross-hybridize with that from the other species, indicating that a great divergence of kDNA sequences has occurred during the evolution of this genus. Comparison of kDNA minicircle sequences of C. fasciculata and C. oncopelti from the literature, showed no significant sequence homology corroborating some of our data. Restriction profiles of kDNA from the different species showed no genotypic similarity thus supporting the status of separate taxa. Furthermore, no cross-hybridization was detected between kDNA from parasites of the genus Crithidia and kDNA from parasites of different genera of monogenetic and digenetic trypanosomatids such as Herpetomonas, Blastocrithidia, Trypanosoma, Leishmania, Endotrypanum and Phytomonas.
