Kinetoplast and Nuclear DNA Polymorphism of Visceral Leishmania Isolates from Northeastern India
1995 年 5 巻 1 号 p. 27-32
詳細
1995 年 5 巻 1 号 p. 27-32
The recent epidemic outbreaks of visceral leishmaniasis have created a major health problem in India. Whether these outbreaks and the drug-resistance seen in about a quarter of these cases are genetically based remains unknown. To facilitate a better understanding of these possibilities, we have begun to analyse kinetoplast (k) and nuclear (n) DNA heterogeneity of Leishmania isolates from Bihar (DD8, RMRI and SS) and West Bengal (AG and UR6). Schizodeme and Southern blot analyses of their kDNA separated these isolates into three groups, i.e. DD8, RMRI and SS; AG and UR6. This grouping is consistent with the results of restriction fragment length polymorphisms seen at the gp63 and β-tubulin loci by Southern blot analysis of nDNA. The results obtained better define the genetic heterogeneity of Indian visceral Leishmania isolates for further characterization of their relationships to virulence and drug-resistance.
