Construction of Polymerase Chain Reaction Primer to Detect Cryptosporidium parvum or C. muris
1995 年 5 巻 4 号 p. 149-156
詳細
1995 年 5 巻 4 号 p. 149-156
Two sets of polymerase chain reaction primers that amplify DNA fragments of Cryptosporidium parvum or C. muris were constructed by adapting a simple method, namely cloning and sequencing of randomly amplified polymorphic DNA (RAPD) of corresponding species. The primers successfully amplified genomic DNA of C. parvum or C. muris as well as sample DNA obtained from feces of a human patient, naturally infected cattle or experimentally infected mice. And we also developed the method to obtain DNA of Cryptosporidium from the oocysts in feces.
