Z-Chromosome Specific Primers for Chicken-Quail Hybrid Blastoderm

Soichi Takagi; Tamao Ono; Akira Tsukada; Yusuke Atsumi; Shusei Mizushima; Noboru Saito; Kiyoshi Shimada

doi:10.2141/jpsa.44.209

Physiology, Reproduction and Immunology: Research Note

Z-Chromosome Specific Primers for Chicken-Quail Hybrid Blastoderm

Soichi Takagi, Tamao Ono, Akira Tsukada, Yusuke Atsumi, Shusei Mizushima, Noboru Saito, Kiyoshi Shimada

Author information

Soichi Takagi
Laboratory of Animal Physiology, Graduate School of Bioagricultural Sciences, Nagoya University
Tamao Ono
Department of Food Production Science, Faculty of Agriculture, Shinshu University
Akira Tsukada
Laboratory of Animal Physiology, Graduate School of Bioagricultural Sciences, Nagoya University
Yusuke Atsumi
Department of Food Production Science, Faculty of Agriculture, Shinshu University
Department of Bioscience and Food Production Science, Interdisciplinary Graduate School of Science and Technology, Shinshu University
Shusei Mizushima
Laboratory of Animal Physiology, Graduate School of Bioagricultural Sciences, Nagoya University
Noboru Saito
Laboratory of Animal Physiology, Graduate School of Bioagricultural Sciences, Nagoya University
Kiyoshi Shimada
Laboratory of Animal Physiology, Graduate School of Bioagricultural Sciences, Nagoya University

Keywords: chicken, PCR, PKCI, quail, Z-chromosome

JOURNAL FREE ACCESS

2007 Volume 44 Issue 2 Pages 209-212

DOI https://doi.org/10.2141/jpsa.44.209

Details

Abstract

A polymerase chain reaction (PCR) primer set for a chicken pkci gene on the Z-chromosome, chPKCI, amplified a 329 bp fragment of genomic DNA in chickens but not quail. Likewise, a PCR primer set for the quail pkci gene on the Z-chromosome, quPKCI, amplified a 95 bp fragment of genomic DNA in quail but not chickens. These chicken and quail primers validly identified chicken-quail hybrids derived from Z-chromosome carrying sperm of chicken and the hybrid derived from Z-chromosome carrying oocyte of quail, respectively.

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