Characteristics of Novel Chicken Embryonic Stem Cells Established Using Chicken Leukemia Inhibitory Factor

Mikiharu Nakano; Kenjiro Arisawa; Satomi Yokoyama; Masaki Nishimoto; Yusuke Yamashita; Maya Sakashita; Ryo Ezaki; Haruo Matsuda; Shuichi Furusawa; Hiroyuki Horiuchi

doi:10.2141/jpsa.010102

This article has now been updated. Please use the final version.

Characteristics of Novel Chicken Embryonic Stem Cells Established Using Chicken Leukemia Inhibitory Factor

Mikiharu Nakano, Kenjiro Arisawa, Satomi Yokoyama, Masaki Nishimoto, Yusuke Yamashita, Maya Sakashita, Ryo Ezaki, Haruo Matsuda, Shuichi Furusawa, Hiroyuki Horiuchi

Author information

Mikiharu Nakano
Department of Molecular and Applied Biosciences, Graduate School of Biosphere Science, Hiroshima University, Japan
Kenjiro Arisawa
Hiroshima Prefectural Institute of Industrial Science and Technology, Hiroshima Industrial Promotion Organization, Japan
Satomi Yokoyama
Department of Molecular and Applied Biosciences, Graduate School of Biosphere Science, Hiroshima University, Japan
Masaki Nishimoto
Hiroshima Prefectural Institute of Industrial Science and Technology, Hiroshima Industrial Promotion Organization, Japan
Yusuke Yamashita
Department of Molecular and Applied Biosciences, Graduate School of Biosphere Science, Hiroshima University, Japan
Maya Sakashita
Department of Molecular and Applied Biosciences, Graduate School of Biosphere Science, Hiroshima University, Japan
Ryo Ezaki
Department of Molecular and Applied Biosciences, Graduate School of Biosphere Science, Hiroshima University, Japan
Haruo Matsuda
Department of Molecular and Applied Biosciences, Graduate School of Biosphere Science, Hiroshima University, Japan
Shuichi Furusawa
Department of Molecular and Applied Biosciences, Graduate School of Biosphere Science, Hiroshima University, Japan
Hiroyuki Horiuchi
Department of Molecular and Applied Biosciences, Graduate School of Biosphere Science, Hiroshima University, Japan
Hiroshima Prefectural Institute of Industrial Science and Technology, Hiroshima Industrial Promotion Organization, Japan

Keywords: Chicken embryonic stem cells, Chicken Nanog, Chicken vasa homolog, Leukemia inhibitory factor, Primordial germ cells

JOURNAL FREE ACCESS Advance online publication

Article ID: 010102

DOI https://doi.org/10.2141/jpsa.010102

The final version of this article is now available: Vol. 48 (2011) , No. 1 p.64

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Abstract

Chickens are an ideal animal model study tool for developmental biology, and a farm animal with excellent productivity. Researchers have therefore long sought to establish chicken embryonic stem cells (cESCs) to enable the creation of genetically modified chickens. Here, we derived novel cESCs from chicken blastodermal cells (CBCs) cultured with chicken leukemia inhibitory factor (chLIF). These cESCs have the capacity for long-term (100 days or more) successive subculture and express both chicken Nanog (chNanog) and chicken vasa homolog (Cvh) mRNAs and proteins. The cESCs showed a capacity for chimeric formation during a transplant experiment that used a fertilized egg. Transfer of the enhanced green fluorescent protein (EGFP) gene to cESCs enabled green fluorescence to be observed among primordial germ cells (PGCs). These results indicate that novel cESCs should have the capacity to differentiate into germ cells.

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