Abstract
The sera from two domestic (DN and DM) and a wild strains and their F1 hybrids (wild×DM) of Japanese quails were examined for amylase isozymes by the use of polyacrylamide gel electrophoresis. The results of this investigation was summarized as follows:
1) Seven anodal bands were detected in total, which were classified into three groups, Regions I, II and III (Fig. 1). Two bands in Region I invariably occurred throughout the materials. Bands in Regions II and III showed variations, but those in Region III had a low activity and were indistinct. Accordingly, two bands occurring in Region II were subjected to genic analysis. Then, 641 birds in total were observed to determine gene frequencies among the strains.
2) With regard to the two bands in Region II, there were three phenotypes AA (a fast band), BB (a slow band) and AB (Fig. 1). They were controlled by autosomal codominant alleles symboled Amy-1A and Amy-1B.
3) In all strains examined, the frequency of Amy-1B was higher than that of Amy-1A. The frequencies of Amy-1A were 7.7% in the wild and 21.7% in the domestic strain.
