The tests were carried out with 10-day-old White Leghorn cockrels maintained free from coccidial infection. During the experiments they were kept in wire-floored metal cages and housed in air-conditioned rooms continuously illuminated. The birds had access to food and water at all times. The birds were divided into groups of 10 birds each which were equally balanced in weight. Unmedicated-infected and unmedicated-uninfected controls of chicks were included in each test. Inoculum containing 50, 000 sporulated oocysts of Eimeria tenella and 100, 000 sporulated oocysts of E. acervulina was administered in a dose of 0.2ml per bird per time. The mixed infection of E. tenella and E. acervulina was conducted once a day for 1, 2, 4 or 6 days continuously. Beclotiamine-NDS (BCT) and sulfachloropyrazine (SCP) were added to the feed at 100ppm and 330ppm level, respectively. The infected birds were medicated for 7 days or 14 days starting from the same time as the initial inoculation of oocyst. Some of the infected birds medicated for 7 days were challenged at 15 days after the first inoculation. All experiments were terminated 28 days after the inoculation.
Evaluation method for anticoccidial efficacy: Body weight, mortality, number of oocysts in feces and coccidial lesions were recorded. The weights were averaged per group. Oocyst counts (OPG) were measured in each group, from 4 days to 28 days after the first inoculation. Lesions were scored by an arbitrary system with 0 to 4 corresponding the negative to the most severe lesion.
The results were as follows.
Mortality: Mortality of the infected controls were 30, 40, 50 and 70% in groups inoculated 1, 2, 4 and 6 times, respectively. Medication of SCP and BCT was effective for preventing chicks from death by coccidiosis.
Infected control groups: Oocyst outputs (OPG) of the infected controls showed peak at the 7th day (E. tenella) and the 5th or 6th day (E. acervulina) after the first inoculation. Total OPOs of E. acervulina were 39.21×10⁵, 44.05×10⁵, 63.34×10⁵ and 56.30×10⁵, and total OPGs of E. tenella were 7.44×10⁵, 11.03×10⁵, 10.65×10⁵ and 11.10×10⁵ in groups inoculated 1, 2, 4 and 6 times, respectively. Oocyst outputs were not observed after challenge with oocyst at the 15th day. The relative weight gains of the infected controls inoculated 1 to 6 times were 87% to 72% during 28 days after the first inoculation.
Groups medicated with BCT for 7 days: Oocyst outputs of E. tenella was completely controlled during medication in groups inoculated 1 to 6 times. After the removal of medication the small size of oocyst output was observed in groups inoculated 4 and 6 times. Medication of BCT against E. acervulina permitted some oocyst production. The small number of oocysts of E. tenella was recorded in the group inoculated 1 time after challenge. Body weight gain of the inoculated 1 to 4 times was similar to that of the uninfected control.
Groups medicated with SCP for 7 days: Oocyst output of E. acervulina was completely controlled during the period of observation. Oocyst production of E. tenella was completely controlled during medication, but, except the group inoculated one time, the small number of oocysts was observed after the withdrawal of medication in the groups inoculated 2, 4 and 6 times. Oocyst output after challenge was detected in the small number of oocysts of E. acervulina in the groups inoculated 1, 2 and 4 times. Excellent relative weight gain of the birds inoculated 1 and 2 times and administered SCP was observed.
Groups medicated with BCT for 14 days: Oocyst output of E. tenella was completely controlled during the period of observation, but oocyst output of E. acervulina was observed in a small amount.

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The sera from two domestic (DN and DM) and a wild strains and their F₁ hybrids (wild×DM) of Japanese quails were examined for amylase isozymes by the use of polyacrylamide gel electrophoresis. The results of this investigation was summarized as follows:
1) Seven anodal bands were detected in total, which were classified into three groups, Regions I, II and III (Fig. 1). Two bands in Region I invariably occurred throughout the materials. Bands in Regions II and III showed variations, but those in Region III had a low activity and were indistinct. Accordingly, two bands occurring in Region II were subjected to genic analysis. Then, 641 birds in total were observed to determine gene frequencies among the strains.
2) With regard to the two bands in Region II, there were three phenotypes AA (a fast band), BB (a slow band) and AB (Fig. 1). They were controlled by autosomal codominant alleles symboled Amy-1^A and Amy-1^B.
3) In all strains examined, the frequency of Amy-1B was higher than that of Amy-1^A. The frequencies of Amy-1^A were 7.7% in the wild and 21.7% in the domestic strain.

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In order to investigate influences of age of growing chicks on the development of intestine, on the frequency of feces excretion and on the amount of rectal and cecal feces, the present experiment was carried out using single comb White Leghorn chicks. The results obtained were summarized as follows.
1) Weight, length and surface area of duodenum, small intestine excluding duodenum, both side of ceca, and rectum were measured at 10-day interval from 10 days to 150 days of age. The values were compared as body weight basis. As a result, the values at 10 days of age showed the largest and thereafter they declined rapidly till 60 days of age, then the values remained almost constant.
2) The 30-days-old chicks excreted 44g of rectal feces a day, and the frequency of fecal excretion was 51 times. While, in the 60-, 90- and 120-days-old chicks the amount of rectal feces and its excretory frequency were twice and two thirds, respectively, that in the 30-days-old chicks. The amount of cecal feces was 6g a day at 30 and 60 days of age and 3-2g at 90 and 120 days of age. The frequency of cecal feces excretion decreased with increasing age from 30 to 120 days. The peak of hourly frequency in excretion of the cecal feces at morning was become smaller with the growth of chicks. Therefore, it is suggested that the amount and the hourly frequency of cecal feces excretion might be affected by age of chicks. (Studies on the function of ceca of chickens. Report 5)

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The collaborative experiment in two laboratoryies was carried out to investigate the effect of dietary protein and energy levels on the abdominal fat content of broilers and the relationship between its content and meat taste.
Chicks were reared on a commercial starter diet (CP22%-TDN72%) for 4 weeks and divided into two groups, such as CP16%-TDN73% and CP20%-TDN73%, up to 8 weeks old. Then each group was divided into 3 groups up to 10 weeks old, the former; CP16%-TDN82%, CP16%-TDN73% and CP20%-TDN73%, the latter; CP 20%-TDN82%, CP20%-TDN73% and CP24%-TDN73%, respectively.
The abdominal fat level was significantly higher in both sexes fed low protein diets than in those fed high protein diets, but female chicks had a significantly higher level of abdominal fat than male chicks.
The level-up of dietary protein by 4% depressed abdominal fat level, but that of TDN by 9% had a little effect compared with that of dietary protein by 4%.
High correlation was recognized between the fat content in thigh muscle and abdominal fat level. (r=0.94)
In sensory test, thigh muscle, pickled in 1% NaCl solution for 5 minites and steamed, was used. Most of panelist liked the meat of broilers fed diets which resulted in high fat contents. Meat taste of broilers may be affected by fat content in muscle.

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Performance of the hens and cocks of five generations on either the control diet or the diet containing 15% of one of four kinds of yeast grown on n-paraffin by four domestic manufacturers was combined and analysed statistically. The meat and eggs of the hens of the third generation fed either the control or yeast diet for longer than two years were analyzed chemically for nucleic acids, heavy metals, i, e. Hg, Pb, Cd and As, and polycyclic aromatic compounds such as benzpyrene, which are suspected to be carcinogenic.
Followings were obtained as conclusion of the experiments reported in this and previous papers:
1. Yeast grown on n-paraffin is an excellent source of protein, energy and phosphorus for poultry, except sulphur amino acids and vitamin B₁₂, of which content in yeast is low for poultry feed, resembling the nutritive nature of soybean meal.
2. No evidence is obtained indicating that the yeast contains such a large quantity of toxic substance that the meat and eggs produced by the yeast feeding are injurious to human health. No data are obtained suggesting that the yeast feeding is unfavorable to produce the meat and eggs of good quality.
3. It is suggested that the yeast contains some vital factor or factors which keeps hens on the yeast healthy and productive.

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During the multi-generation feeding experiment on yeast grown on n-paraffin summarized and reported previously1), samples for histopathological examination were taken from 25 parts of the organs of 120 hens of the first generatin. Details in the changes observed on the preparation will be published soon in this Journal2).
In this paper, are presented statistical analyses on the changes of the hens on either the control or yeast diet.

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