Abstract
We tried to establish the microplate enzyme linked immunosolvent assay (ELISA) which detected antibodies derived from avian leukosis virus (ALV) infection by using Rous associated virus type 2 (RAV-2) antigen for coating, on purpose to apply the ELISA system to ALV monitoring system of specific pathogen free (SPF) chicken.
Using 3 antigens (RAV-1: subgroup A (A), RAV-2: subgroup B (B), normal cell (N)), ELISA was applied for the sera of SPF chicken which had been already demonstrated that the serum neutralization (SN) value was less than 5. All of the optical density (OD) value of the sera were less than 0.3. Both differences of OD value (A-N, B-N) were less than 0.05.
When ELISA was also applied for the sera of chicken which had been infected avian sarcoma-leukosis viruses, the relationship between OD value differences of A-N and B -N was correlative. So, the B antigen was used for coating antigen, because it was easy for preparing.
ELISA titer of our standard positive serum for antibody measurement was decided to 128, 000. The titer of serum sample was calculated by comparing the OD value with standard curve of the standard positive serum.
It seems that our established method is effective for measurement of ALV antibody of SPF chicken serum.
