Comparing of Enzyme Linked Immunosolvent Assay, Serum Neutralization and Fluorescent Antibody Method for Measuring the Avian Leukosis Virus Antibody

Abstract

We analyzed the antigen-antibody reaction system of enzyme linked immunosolvent assay (ELISA) which measured the avian leukosis virus (ALV) antibody, and compared the correlations and the sensitivity among three methods (ELISA, serum neutralization: SN, fluorescent antibody: FA).
(1) The sera of chickens experimentally infected with subgroup A and B viruses were investigated using 3 methods (ELISA, FA and SN). It seemed that ELISA and FA detected mainly group specific (gs) antibodies, but SN detected subgroup specific antibodies.
(2) Three kinds of antigen components were confirmed to produce gs antibodies and the order of appearance of gs antibodies after infection was anti-p27, anti-gp 85 and anti-p15.
(3) There were correlations among antibody titers of ELISA, FA and SN.
(4) The sensitivities of three methods were compared; SN and FA had about same sesitivity, but ELISA was about 10 times more sensitive than SN and FA.