Abstract
It was first reported by LANDSTEINER AND MILLER (1924) that the blood types of chicken were classified into eight different antigens by using anti-chicken red cells immune rabbit sera absorbed with various chicken red cells.
Afterwards many studies have been carried out. Recentry twelve different blood group systems were classified in red cells of chickens by iso-immune agglutinins.
The present experiments were performed for the classification of chickens red cells by using natural isoagglutinins.
The chicken red cells were treated with papain enzyme solution. The results obtained were as follows:
1) Two typical agglutinins (anti-FsI and anti-FsII) were found in the chicken normal sera and two agglutinogens (FsI and FsII) corresponding with each agglutinin were present.
2) The blood of chickens was classified into three types, FsI, FsII and Fs0.
3) The agglutinin-titers of anti-FsI and anti-FsII sera were 1:8, respectively.
4) The antibodies (anti-FsI and anti-FsII) were absorbed to volume of 16 fold antigen (FsI and FsII).
5) The antibodies were eluted from antigen-antibodies complex which anti-FsI and anti-FsII sera were combined with FsI, FsII red cells.
6) The presence of antigenicity in skin and the basal part of quill was clarified in chickens by elution test.
