日本薬理学会年会要旨集
Online ISSN : 2435-4953
第93回日本薬理学会年会
セッションID: 93_3-O-127
会議情報

一般演題(口頭)
CBIS法を用いた抗ウマポドプラニンモノクローナル抗体の樹立
*佐山 勇輔金子 美華加藤 幸成
著者情報
キーワード: monoclonal antibody
会議録・要旨集 オープンアクセス

詳細
抄録

Purpose: Podoplanin is expressed in normal tissues including renal podocytes and lymphatic endothelial cells. To investigate the expression and function of horse podoplanin (horPDPN), sensitive and specific mAbs against horPDPN are necessary. In this study, we aimed to develop useful anti-horPDPN mAbs for many applications such as flow cytometry (FCM), western blot (WB), and immunohistochemistry (IHC).

Methods: We employed a conventional immunization method using synthetic peptides or Cell-Based Immunization and Screening (CBIS) method using horPDPN-expressed mammalian cells for producing anti-horPDPN mAbs. Anti-horPDPN mAbs were screened using enzyme-linked immunosorbent assay or FCM. Established anti-horPDPN mAbs were characterized using FCM, WB, and IHC.

Results: We developed two anti-horPDPN mAbs, PMab-202 using the peptide immunization and PMab-219 using CBIS method. PMab-202 reacted with horPDPN in FCM and WB, but did not stain horPDPN in IHC. In contrast, PMab-219 detected horPDPN in not only FCM and WB, but also IHC.

Conclusion: We have successfully established mouse anti-horPDPN mAbs, PMab-202 and PMab-219. PMab-219 is applicable for FCM, WB, and IHC analyses. CBIS method could be more advantageous to establish immunohistochemistry-applicable mAbs for elucidating the pathophysiological function of horPDPN.

著者関連情報
© 2020 本論文著者
前の記事 次の記事
feedback
Top