マウス脳内出血病態に対するモノスルフィドおよびトリスルフィドの作用

抄録

Intracranial hemorrhage (ICH) is a type of hemorrhagic strokes that is caused due to bleeding in the brain parenchyma. Here we investigated the effects of Na₂S and Na₂S₃ on ICH in mice. Na₂S and Na₂S₃ were injected into two separate groups of mice (25 µmol/kg i.p.) 30 min before induction of ICH by collagenase injection into the striatum. Both agents attenuated the neutrophil infiltration (MPO), inhibited the upregulation of monocyte/macrophage chemokine (CCL-2) expression and maintained the axonal fibers transport function (APP). Moreover, pretreatment with Na₂S improved the motor function, prevented the decrease in the neuronal count (NeuN), maintained the integrity of axonal fiber structures (neurofilament-H) and lowered the upregulation of neutrophil chemokine (CXCL-2) expression after induction of ICH. On the other hand, pretreatment with Na₂S₃ significantly attenuated the activation of microglia/macrophages (Iba-1) in the perihematomal area. Both agents failed to prevent ICH-induced increase in the brain vascular permeability or to lower the pro-inflammatory cytokine (IL-6) expression significantly. In conclusion, these results suggest that Na₂S exhibits more potent neuroprotective effect than Na₂S₃ and promotes recovery of neurological functions after ICH.