生体模倣システムを用いたがん微小環境モデル内の血管網の作出と介入

抄録

The tumor microenvironment, including the vascular network and blood flow, influence cancer cell behaviors in a tumor. To accurately replicate this environment in vitro, we developed a tumor model with a perfusable vascular network. We incorporated tumor spheroids or colorectal cancer-derived organoids into a microfluidic device [1-2]. Angiogenic secretions from the tumor models facilitated the formation of the vascular network. After integrating with a vascular network, drugs were administered through the network, and we assessed cellular responses using immunostaining and oxygen metabolism analysis. The vascular network successfully perfused the tumor models for at least four days, promoting their proliferation and preventing cell death. We observed different drug sensitivities between static and perfusion conditions. Our electrochemical method effectively quantified the oxygen metabolism of the cancer organoids and revealed differences in drug sensitivity [2]. This innovative approach holds great promise for advancing cancer models, including selectively targeting specific subpopulations within a tumor [3].

References: [1] Y. Nashimoto et al., Biomaterials, 229, 119547 (2020). [2] Y. Nashimoto et al., Biosens. Bioelecton., 219, 114808 (2023). [3] Y. Nashimoto et al., Front. Bioeng. Biotechnol., 11, 1184325 (2023).