Abstract
[Purpose] The purpose of the present study was to investigate the effect of magnetic infrared laser (MIL) radiation on the xylene-induced acute inflammation. [Subjects] The subject animals were male ICR mice. [Methods] Mice were once irradiated (60s) by MIL at 1.33, 2.66 or 6.65 J/cm2 of MIL, or treated with indomethacin or dexamethasone. Then xylene (0.03 ml) was topically applied to the anterior surface of the right ear to induced inflammation 1 h after irradiation with MIL. The changes in ear weights, histological profiles and histomorphometrical measurements of the ear were conducted upon sacrifice. All animals were sacrificed 2 h after xylene application. Results of MIL irradiation were compared to those of indomethacin and dexamethasone (15 mg/kg injected once intraperitoneally). [Results] Xylene application resulted in marked increases in xylene-insulted ear weights compared to that of the intact control. Thus, the differences between intact and insulted ears were also significantly increased. The histological characteristics of acute inflammation, such as severe vasodilation and edematous changes of skin, were detected in xylene-treated control ears with marked increase in the thickness of the ear tissues. However, these xylene-induced acute inflammatory changes were significantly and dose-dependently decreased by MIL irradiation. [Conclusion] We conclude that MIL therapy has a favorable effect in the reduction of the acute inflammatory responses induced by xylene application in mice.
