Journal of Physical Therapy Science
Online ISSN : 2187-5626
Print ISSN : 0915-5287
ISSN-L : 0915-5287
Original Articles
Hypokinesia Causes Oxidative Damage and Mitochondrial DNA Deletion in Rat Skeletal Muscle
Yasutomo SakaiYukio IwamuraHiroshi NagataNao YamamotoNorio OhkoshiShintaro Ueda
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1997 Volume 9 Issue 2 Pages 103-109


The present study was conducted to determine effects of oxidative stress on mitochondrial genome caused by disuse atrophy of skeletal muscles by hindlimbs suspension. We used rats as subjects, and produced hypokinesia by hindlimbs’ (tail) suspension. With electron microscopy, confirming previous results, we found mitochondrial swelling, accompanied by blurred matrix changes in the soleus muscle. Surprisingly, in these muscles, we were able to detect two types of large-scale deletions (about 3.9 and 5.2 kb) of mitochondrial DNA (mtDNA) by the polymerase chain reaction. Our study showed that hypokinesia for two weeks caused not only oxidative damage to the histology of skeletal muscular mitochondoria and but also deletion of mtDNA. Situated close to the energy-generating system, mtDNA is susceptible to oxidative stress and is likely to generate mutations.

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© 1997 by the Society of Physical Therapy Science
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