Zinc supplementation during in vitro maturation increases the production efficiency of cloned pigs

Yubyeol JEON; Junchul David YOON; Lian CAI; Seon-Ung HWANG; Eunhye KIM; Eunsong LEE; Eui Bae JEUNG; Sang-Hwan HYUN; Woo Suk HWANG

doi:10.1262/jrd.2016-072

This article has now been updated. Please use the final version.

Zinc supplementation during in vitro maturation increases the production efficiency of cloned pigs

Yubyeol JEON, Junchul David YOON, Lian CAI, Seon-Ung HWANG, Eunhye KIM, Eunsong LEE, Eui Bae JEUNG, Sang-Hwan HYUN, Woo Suk HWANG

Author information

Yubyeol JEON
Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea
Sooam Biotech Research Foundation, Seoul 08359, Republic of Korea
Junchul David YOON
Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea
Lian CAI
Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea
Seon-Ung HWANG
Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea
Eunhye KIM
Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea
Eunsong LEE
Laboratory of Theriogenology, College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Republic of Korea
Eui Bae JEUNG
Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea
Sang-Hwan HYUN
Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea
Woo Suk HWANG
Sooam Biotech Research Foundation, Seoul 08359, Republic of Korea

Keywords: Cloning efficiency, In vitro maturation (IVM), Porcine, Somatic cell nuclear transfer (SCNT), Zinc

JOURNAL FREE ACCESS Advance online publication

Article ID: 2016-072

DOI https://doi.org/10.1262/jrd.2016-072

The final version of this article is now available: Vol. 62 (2016), No. 6 pp. 635-638

Details

Abstract

Zinc supplementation (0.8 µg/ml) in in vitro maturation (IVM) medium significantly enhances oocyte quality. In this study, we compared the development of somatic cell nuclear transfer (SCNT) embryos produced from conventional IVM (control) and zinc-supplemented IVM oocytes. A total of 1206 and 890 SCNT embryos were produced using control and zinc-supplemented oocytes, respectively, and then were transferred to 11 and 8 recipients, respectively. Five control recipients and three zinc-supplemented recipients became pregnant. Two live piglets and eight mummies were born from two control recipients, and ten live piglets and six stillborn piglets were born from three zinc-supplemented recipients. The production efficiency significantly increased in the zinc-supplemented group (0.33% vs. 3.02%). This report suggests that zinc supplementation in IVM medium improved the production efficiency of cloned pigs.

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