Journal of Radiation Research
Online ISSN : 1349-9157
Print ISSN : 0449-3060
Unstable Structure of Ribosomal Particles Synthesized in γ-irradiated Escherichia coli
藤田 斉森田 健二
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1975 年 16 巻 2 号 p. 113-119

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Stability of Escherichia coli ribosomes newly synthesized after γ-irradiation was compared with that of normal ribosomes. The ribosomal particles around 70-S synthesized in irradiated cells were more sensitive to digestion by pancreatic ribonuclease A. There existed a higher amount of the salt-unstable ‘50-S’ precursor particles in the extract from irradiated cells than in the extract from unirradiated cells. These facts suggest that ribosomal particles synthesized during an earlier stage in irradiated cells maintain an incomplete structure, even though they are not distinguishable from the normal ribosomes by means of sucrose density-gradient centrifugation.
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