抄録
A bacterial plasmid gene mucAB has an error-prone activity and is widely used for sensitive detection of mutagens as Ames test. The mucAB gene (1.7 Kbp) was cloned into a vector carrying the mammalian metallothionein promoter (pTE40), which enhanced transformation of cultured mouse cells in the previous study. In this study, mucAB was introduced into an embryo of C57Bl mouse to obtain mucAB transgenic mice. Among 93 mice from 439 injections, we found only one mice in which genome mucAB was integrated. Heterogenic mucAB mice from this founder were examined for tumor induction efficiency by subcutaneous injection of 0.02 mg methylchoranthrene. The mice developed fibrosarcomas with an elevated efficiency (24%) as compared with control mice without mucAB (8%) until the present moment. [J Radiat Res 44:397 (2003)]
