抄録
UV-B easily produces cyclobutane pyrimidine dimers (CPD) and (6-4) photoproducts. They inhibit cellular and physiological activity and impair genetic stability. Responses of plants to UV-B varies among species and cultivars. Cucumber and spinach are sensitive to UV-B. Hypersensitivity of a rice cultivar to UV-B has been reported to be due to reduced photoreactivation (PR). We have previously reported a nucleotide sequence of a gene for CPD photolyase in spinach. The gene complemented E. coli deficient in PR. We here further investigated a gene for spinach CPD photolyase. E. coli deficient in PR was transformed with the cloned gene and the content of CPD was determined by ELISA mehtod. The extent of repair of CPD was much higher in E. coli treated with visible light than untreated control. The gene has been reported to highly express in flower in Arabidopsis. However in spinch the expression in leaf was much higher than other organs, root, stem and flower. [J Radiat Res 44:413 (2003)]
